Journal: Bioscience Reports

Article Title: Identification of key genes of papillary thyroid carcinoma by integrated bioinformatics analysis

doi: 10.1042/BSR20201555

Figure Lengend Snippet: PCR primers

Article Snippet: PDVF membrane was blocked and then incubated with primary anti-ADORA1 antibodies (1:500 dilution, Bioss, bs-6649R), APOE (1:1500 dilution, Bioss, bs-4892R), LPAR5 antibodies (1:200 dilution, Bioss, bs-15366R), and β-actin (1:5000 dilution, Bioss, bs-0061R) at 4°C overnight followed by incubation with secondary antibodies (Zhongshanjinqiao) (1:1000 dilution) at 37°C for 1 h. The signal was detected using ECL method.

Techniques: Sequencing

Journal: Bioscience Reports

Article Title: Identification of key genes of papillary thyroid carcinoma by integrated bioinformatics analysis

doi: 10.1042/BSR20201555

Figure Lengend Snippet: ADORA1, APOE, CCL13, CDH2, CXCL12, EVA1A, FAM20A, FN1, GNAI1, LPAR5, MFGE8, NMU, SERPINA1, TIMP1, and TNC are overexpressed in PTC tissues compared with paracancerous tissue, while GNAI and GPC3 are down-regulated.

Article Snippet: PDVF membrane was blocked and then incubated with primary anti-ADORA1 antibodies (1:500 dilution, Bioss, bs-6649R), APOE (1:1500 dilution, Bioss, bs-4892R), LPAR5 antibodies (1:200 dilution, Bioss, bs-15366R), and β-actin (1:5000 dilution, Bioss, bs-0061R) at 4°C overnight followed by incubation with secondary antibodies (Zhongshanjinqiao) (1:1000 dilution) at 37°C for 1 h. The signal was detected using ECL method.

Techniques:

Journal: Bioscience Reports

Article Title: Identification of key genes of papillary thyroid carcinoma by integrated bioinformatics analysis

doi: 10.1042/BSR20201555

Figure Lengend Snippet: Expression levels of ADORA1, APOE, C5AR1, EVA1A, FAM20A, GNAI1, LPAR5, MFGE8, OPRM1, SERPINA1, SSTR3, and TIMP1 are related to the overall survival of patients with PTC.

Article Snippet: PDVF membrane was blocked and then incubated with primary anti-ADORA1 antibodies (1:500 dilution, Bioss, bs-6649R), APOE (1:1500 dilution, Bioss, bs-4892R), LPAR5 antibodies (1:200 dilution, Bioss, bs-15366R), and β-actin (1:5000 dilution, Bioss, bs-0061R) at 4°C overnight followed by incubation with secondary antibodies (Zhongshanjinqiao) (1:1000 dilution) at 37°C for 1 h. The signal was detected using ECL method.

Techniques: Expressing

Journal: Bioscience Reports

Article Title: Identification of key genes of papillary thyroid carcinoma by integrated bioinformatics analysis

doi: 10.1042/BSR20201555

Figure Lengend Snippet: Expression levels of ADCY8, ADORA1, APOE, CHGB, FN1, LPAR5, NMU, and TNC are significantly related to the disease-free survival of patients with PTC.

Article Snippet: PDVF membrane was blocked and then incubated with primary anti-ADORA1 antibodies (1:500 dilution, Bioss, bs-6649R), APOE (1:1500 dilution, Bioss, bs-4892R), LPAR5 antibodies (1:200 dilution, Bioss, bs-15366R), and β-actin (1:5000 dilution, Bioss, bs-0061R) at 4°C overnight followed by incubation with secondary antibodies (Zhongshanjinqiao) (1:1000 dilution) at 37°C for 1 h. The signal was detected using ECL method.

Techniques: Expressing

Journal: Bioscience Reports

Article Title: Identification of key genes of papillary thyroid carcinoma by integrated bioinformatics analysis

doi: 10.1042/BSR20201555

Figure Lengend Snippet: ( A–C ) Validation of expression levels of ADORA1, APOE, and LPAR5 by RT-qPCR in 30 cases of PTC and matched adjacent tissues. ( D ) ADORA1, APOE, and LPAR5 protein levels are increased in four cases of PTC and matched adjacent tissues, as measured by Western Blot; *** P <0.001.

Article Snippet: PDVF membrane was blocked and then incubated with primary anti-ADORA1 antibodies (1:500 dilution, Bioss, bs-6649R), APOE (1:1500 dilution, Bioss, bs-4892R), LPAR5 antibodies (1:200 dilution, Bioss, bs-15366R), and β-actin (1:5000 dilution, Bioss, bs-0061R) at 4°C overnight followed by incubation with secondary antibodies (Zhongshanjinqiao) (1:1000 dilution) at 37°C for 1 h. The signal was detected using ECL method.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Bioscience Reports

Article Title: Identification of key genes of papillary thyroid carcinoma by integrated bioinformatics analysis

doi: 10.1042/BSR20201555

Figure Lengend Snippet: ( A ) Relative methylation level of ADORA1, APOE and LPAR5 based on normal thyroid tissues and PTC tissues, individual cancer stages and nodal metastasis status, respectively (UALCAN). ( B ) The correlation between the three genes and TIICs (TIMER); TIICs, tumor infiltrating immune cells.

Article Snippet: PDVF membrane was blocked and then incubated with primary anti-ADORA1 antibodies (1:500 dilution, Bioss, bs-6649R), APOE (1:1500 dilution, Bioss, bs-4892R), LPAR5 antibodies (1:200 dilution, Bioss, bs-15366R), and β-actin (1:5000 dilution, Bioss, bs-0061R) at 4°C overnight followed by incubation with secondary antibodies (Zhongshanjinqiao) (1:1000 dilution) at 37°C for 1 h. The signal was detected using ECL method.

Techniques: Methylation